Expeditious Extraction of Histones from Limited Cells or Tissue Samples and Quantitative Top-Down Proteomic Analysis

Expeditious Extraction of Histones from Limited Cells or Tissue Samples and Quantitative Top-Down Proteomic Analysis

Histones are the first protein element of chromatin and are concerned in just about all DNA-templated processes. Histones are abundantly post-translationally modified by quite a lot of chromatin-modifying equipment. These post-translational modifications (PTMs) are acknowledged by a variety of “reader” proteins, which recruit further proteins to particular places on chromatin and impart exact and highly effective results on gene regulation. Every PTM sometimes exerts a constructive or unfavorable impact on transcription, and up to date research have proven that histone PTMs operate in a combinatorial histone code: that’s, histone PTMs operate together to exert exact DNA-templated regulation. Thus, there’s a must determine and perceive proteoforms, or unambiguously outlined single protein molecules with all mixtures of modifications.

High-down proteomics is at present the one viable strategy for figuring out and quantitating histone proteoforms, and mass spectrometry devices have turn into sufficiently highly effective to carry out these quantitative analyses in a strong and high-throughput style. These latest improvements have enabled new experimental instructions in chromatin analysis however have additionally launched temporal and different constraints. This has led us to develop the protocols described right here, which enhance throughput, cut back pattern necessities, and keep sturdy quantitation. Though initially designed for high-throughput quantitative top-down proteomics, the protocols described listed below are helpful for a variety of chromatin biology purposes.

Beginning with small quantities of cells or tissue, we describe two fundamental protocols for exceptionally fast and environment friendly nuclei isolation, acid extraction of histones, and high-performance liquid chromatography fractionation of histones into histone households. We moreover describe the quantitative top-down proteomic evaluation of histone H4 proteoforms. © 2021 Wiley Periodicals LLC. Fundamental Protocol 1: Nuclei isolation and acid extraction of histones from mammalian cells in tradition/tissues Fundamental Protocol 2: HPLC fractionation of histones and histone H4 HPLC-MS/MS Assist Protocol: Preparation of intact H3 histone tails by Glu-C digestion

 

Evaluation of 1508 Plasma Samples by Capillary-Move Knowledge-Impartial Acquisition Profiles Proteomics of Weight Loss and Upkeep

Complete, excessive throughput evaluation of the plasma proteome has the potential to allow holistic evaluation of the well being state of a person. Based mostly on our personal expertise and the analysis of latest large-scale plasma mass spectrometry (MS) primarily based proteomic research, we recognized two excellent challenges: gradual and delicate nano-flow liquid chromatography (LC) and irreproducibility of identification of data-dependent acquisition (DDA). We decided an optimum answer decreasing these limitations with sturdy capillary-flow data-independent acquisition (DIA) MS. This platform can measure 31 plasma proteomes per day.
Utilizing this setup, we acquired a large-scale plasma examine of the eating regimen, weight problems and genes dietary (DiOGenes) comprising 1508 samples. Proving the robustness, the entire acquisition was achieved on a single analytical column. Completely, 565 proteins (459 recognized with two or extra peptide sequences) had been profiled with 74% information set completeness. On common 408 proteins (5246 peptides) had been recognized per acquisition (319 proteins in 90% of all acquisitions). The workflow reproducibility was assessed utilizing 34 high quality management swimming pools acquired at common intervals, leading to 92% information set completeness with CVs for protein measurements of 10.9%.
The profiles of 20 apolipoproteins may very well be profiled revealing distinct adjustments. The load loss and weight upkeep resulted in sustained results on low-grade irritation, in addition to steroid hormone and lipid metabolism, indicating useful results. Comparability to different large-scale plasma weight reduction research demonstrated excessive robustness and high quality of biomarker candidates recognized. Monitoring of nonenzymatic glycation indicated a delayed, slight discount of glycation within the weight upkeep section. Utilizing stable-isotope-references, we might instantly and completely quantify 60 proteins within the DIA. In conclusion, we current herein the primary large-scale plasma DIA examine and one of many largest scientific analysis proteomic research to this point. Utility of this quick and sturdy workflow has nice potential to advance biomarker discovery in plasma.
Expeditious Extraction of Histones from Limited Cells or Tissue Samples and Quantitative Top-Down Proteomic Analysis

Comparability of Proteomic Applied sciences for Blood-Based mostly Detection of Colorectal Most cancers

Blood-based protein biomarkers are more and more being explored as supplementary or environment friendly alternate options for population-based screening of colorectal most cancers (CRC). The target of the present examine was to check the diagnostic potential of proteins measured with completely different proteomic applied sciences. The concentrations of protein biomarkers had been measured utilizing proximity extension assays (PEAs), liquid chromatography/a number of response monitoring-mass spectrometry (LC/MRM-MS) and quantibody microarrays (QMAs) in plasma samples of 56 CRC sufferers and 99 individuals freed from neoplasms. In one other strategy, proteins had been measured in serum samples of 30 CRC circumstances and 30 individuals freed from neoplasm utilizing immunome full-length purposeful protein arrays (IpAs).
From all of the measurements, 9, 6, 35 and 14 protein biomarkers overlapped for comparative analysis of (a) PEA and LC/MRM-MS, (b) PEA and QMA, (c) PEA and IpA, and (d) LC/MRM-MS and IpA measurements, respectively. Correlation evaluation was carried out, together with calculation of the world below the curve (AUC) for assessing the diagnostic potential of every biomarker. DeLong’s take a look at was carried out to evaluate the variations in AUC.
AGAROSE MS, PCR SCREENING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE
AGR-MS-1001 1/pk
EUR 2038
Description: Bioscience Mol Bio; Agarose
LC-LC(+)-Biotin
abx184150-5g 5 g
EUR 996
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Human Osteonectin (ON) ELISA Kit
DLR-ON-Hu-48T 48T
EUR 479
  • Should the Human Osteonectin (ON) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Osteonectin (ON) in samples from serum, plasma, tissue homogenates or other biological fluids.
Human Osteonectin (ON) ELISA Kit
DLR-ON-Hu-96T 96T
EUR 621
  • Should the Human Osteonectin (ON) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Osteonectin (ON) in samples from serum, plasma, tissue homogenates or other biological fluids.
Mouse Osteonectin (ON) ELISA Kit
DLR-ON-Mu-48T 48T
EUR 489
  • Should the Mouse Osteonectin (ON) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Osteonectin (ON) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Mouse Osteonectin (ON) ELISA Kit
DLR-ON-Mu-96T 96T
EUR 635
  • Should the Mouse Osteonectin (ON) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Osteonectin (ON) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Rat Osteonectin (ON) ELISA Kit
DLR-ON-Ra-48T 48T
EUR 508
  • Should the Rat Osteonectin (ON) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Osteonectin (ON) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Rat Osteonectin (ON) ELISA Kit
DLR-ON-Ra-96T 96T
EUR 661
  • Should the Rat Osteonectin (ON) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Osteonectin (ON) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Human Osteonectin (ON) ELISA Kit
RDR-ON-Hu-48Tests 48 Tests
EUR 500
Human Osteonectin (ON) ELISA Kit
RDR-ON-Hu-96Tests 96 Tests
EUR 692
Mouse Osteonectin (ON) ELISA Kit
RDR-ON-Mu-48Tests 48 Tests
EUR 511
Mouse Osteonectin (ON) ELISA Kit
RDR-ON-Mu-96Tests 96 Tests
EUR 709
Rat Osteonectin (ON) ELISA Kit
RDR-ON-Ra-48Tests 48 Tests
EUR 534
Rat Osteonectin (ON) ELISA Kit
RDR-ON-Ra-96Tests 96 Tests
EUR 742
Human Osteonectin (ON) ELISA Kit
RD-ON-Hu-48Tests 48 Tests
EUR 478
Human Osteonectin (ON) ELISA Kit
RD-ON-Hu-96Tests 96 Tests
EUR 662
Mouse Osteonectin (ON) ELISA Kit
RD-ON-Mu-48Tests 48 Tests
EUR 489
Mouse Osteonectin (ON) ELISA Kit
RD-ON-Mu-96Tests 96 Tests
EUR 677
Rat Osteonectin (ON) ELISA Kit
RD-ON-Ra-48Tests 48 Tests
EUR 511
Rat Osteonectin (ON) ELISA Kit
RD-ON-Ra-96Tests 96 Tests
EUR 709
Trypsin, Recombinant, Proteomics grade
P1228-1000
EUR 196
Trypsin, Recombinant, Proteomics grade
P1228-10000
EUR 1224
Trypsin, Recombinant, Proteomics grade
P1228-5000
EUR 615
HemoVoid™ Kit
HVK-05 5 Preps
EUR 400
Description: Hemoglobin Removal Kit
HemoVoid™ Kit
HVK-10 10 preps
EUR 587
Description: Hemoglobin Removal Kit
HemoVoid™ Kit
HVK-100 100 preps
EUR 2983
Description: Hemoglobin Removal Kit
HemoVoid™ Kit
HVK-50 50 preps
EUR 1846
Description: Hemoglobin Removal Kit
Biotin-LC-LC-NHS
2346-1000
EUR 1675
Biotin-LC-LC-NHS
2346-250
EUR 588
Biotin-LC-LC-NHS
2346-50
EUR 196
Biotin-LC-LC-NHS
2346-5000 Ask for price
HemoVoid Binding Buffer HVBB
HVBB-1000 1000 mL
EUR 456
Description: HemoVoid™ Buffer
HemoVoid Binding Buffer HVBB
HVBB-500 500 mL
EUR 245
Description: HemoVoid™ Buffer
HemoVoid Buffer Kit - 1000
HVBK-1000 1000 mL
EUR 1166
Description: HemoVoid™ Buffer
HemoVoid Buffer Kit - 500
HVBK-500 500 mL
EUR 615
Description: HemoVoid™ Buffer
HemoVoid Elution Buffer HVEB
HVEB-1000 1000 mL
EUR 456
Description: HemoVoid™ Buffer
HemoVoid Elution Buffer HVEB
HVEB-500 500 mL
EUR 245
Description: HemoVoid™ Buffer
HemoVoid Wash Buffer HVWB
HVWB-1000 1000 mL
EUR 456
Description: HemoVoid™ Buffer
HemoVoid Wash Buffer HVWB
HVWB-500 500 mL
EUR 245
Description: HemoVoid™ Buffer
Biotinylated Bovine Serum Albumin (Biotin-LC-BSA) (3 biotin/BSA)
7097-25
EUR 222
Biotinylated Bovine Serum Albumin (Biotin-LC-BSA) (3 biotin/BSA)
7097-5
EUR 115
Biotinylated Bovine Serum Albumin (Biotin-LC-BSA) (5 biotin/BSA)
7098-25
EUR 233
Biotinylated Bovine Serum Albumin (Biotin-LC-BSA) (5 biotin/BSA)
7098-5
EUR 120
Biotinylated Bovine Serum Albumin (Biotin-LC-BSA) (12 biotin/BSA)
7099-25
EUR 244
Biotinylated Bovine Serum Albumin (Biotin-LC-BSA) (12 biotin/BSA)
7099-5
EUR 131
101Bio Protein Solubilization Reagent for MS
P5W11 NULL
EUR 0
Micro Bead Sterlizer
B1201-E 1 PC
EUR 542.7
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Micro Bead Sterlizer
B1202-E 1 PC
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MS-1020
1911-1
EUR 164
Ms-275
20-abx182582
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MS 275
abx076834-1mg 1 mg
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MS-1020
GL5031-1MG 1 mg
EUR 160
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B1201-BEAD 1 PC
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LC-Protein Kinase G Substrate; LC-Gsubtide (Biotin)
20-abx265657
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  • EUR 773.00
  • EUR 356.00
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ELISA kit for Human MS (Melatonin Sulfate)
E-EL-H1726 1 plate of 96 wells
EUR 534
  • Gentaur's MS ELISA kit utilizes the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human MS. During the reaction, Human MS in the sample or standard competes with a fixed amount of Human MS on the sol
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Description: A competitive ELISA kit for quantitative measurement of Human MS (Melatonin Sulfate) in samples from Serum, Plasma, Cell supernatant
ELISA kit for Human Melatonin sulfate (MS)
KTE61523-48T 48T
EUR 354
Description: Quantitative sandwich ELISA for measuring Human Melatonin sulfate (MS) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human Melatonin sulfate (MS)
KTE61523-5platesof96wells 5 plates of 96 wells
EUR 2252
Description: Quantitative sandwich ELISA for measuring Human Melatonin sulfate (MS) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human Melatonin sulfate (MS)
KTE61523-96T 96T
EUR 572
Description: Quantitative sandwich ELISA for measuring Human Melatonin sulfate (MS) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Kininogen LC antibody
23031-100ul 100ul
EUR 390
Sulfo-LC-SPDP
2325-250
EUR 1567
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2325-50
EUR 436
Biotin-LC-NHS
2345-1000
EUR 1001
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EUR 419
Biotin-LC-NHS
2345-50
EUR 142
Biotin-LC-Kemptide
5-00800 4 x 1mg Ask for price
CD49f LC antibody
70R-49925 100 ul
EUR 244
Description: Purified Polyclonal CD49f LC antibody
CD49e LC antibody
70R-49932 100 ul
EUR 244
Description: Purified Polyclonal CD49e LC antibody
CD49e LC Antibody
20-abx009097
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
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20-abx009102
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sulfo-LC-(+)-Biotin
20-abx185570
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  • EUR 1261.00
  • EUR 578.00
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  • 1 g
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LC-(+)-Biotin hydrazide
abx185659-1g 1 g
EUR 648
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LC-NHS-(+)-Biotin
20-abx185861
  • EUR 634.00
  • EUR 398.00
  • 1 g
  • 250 mg
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CD224 LC Antibody
20-abx121054
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  • EUR 439.00
  • EUR 189.00
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Enteropeptidase LC Antibody
20-abx121089
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MASP1 LC Antibody
20-abx121154
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Myeloperoxidase LC Antibody
20-abx121172
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  • EUR 439.00
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sulfo-LC-SPDP
ADC-L-133 unit Ask for price
NHS-LC-Biotin
A8004-100 100 mg
EUR 119
Description: NHS-LC-biotin (succinimidyl-6-(biotinamindo)hexanoate), also known as NHS-X-biotin is a derivative of D-biotin, a amine-reactive biotinylation agent, that contains a spacer arm off the valeric acid side chain of D-biotin with an NHS ester group at its end.
NHS-LC-Biotin
A8004-1000 1 g
EUR 548
Description: NHS-LC-biotin (succinimidyl-6-(biotinamindo)hexanoate), also known as NHS-X-biotin is a derivative of D-biotin, a amine-reactive biotinylation agent, that contains a spacer arm off the valeric acid side chain of D-biotin with an NHS ester group at its end.
Iodoacetyl-LC-Biotin
A8009-100 100 mg
EUR 264
Description: Iodoacetyl-LC-Biotin (N-iodoacetyl-N-biotinylhexylenediamine), a sulfhydryl-reactive biotinylation agent, is a water-insoluble reagent that requires the dissolution of suitable solvents, including dimethyl sulfoxide (DMSO) and dimethylformamide (DMF).
Biotin LC hydrazide
HY-101885 5mg
EUR 119
pPINK- LC Plasmid
PVT4035 2 ug
EUR 325
HemoVoid™ - Blood Card Reagent Kit
HVBC-10 10 preps
EUR 552
Description: Hemoglobin Removal Kit
HemoVoid™ - Blood Card Reagent Kit
HVBC-100 100 preps
EUR 2937
Description: Hemoglobin Removal Kit
HemoVoid™ - Blood Card Reagent Kit
HVBC-50 50 preps
EUR 1770
Description: Hemoglobin Removal Kit
BrightGreen miRNA qPCR MasterMix-No Dye
MasterMix-mS 4 x 1.25 ml for 500 reactions
EUR 151
Bangs Lab Bead Solution
SOLN1-1000 1000 ML
EUR 155.06
Description: Bangs Lab Bead Solution is ready-to-use solution and is suitable for dilution and/or storage of plain, dyed, or functionalized polymer microspheres
Bangs Lab Bead Solution
SOLN1-2000 2000 ML
EUR 212.7
Description: Bangs Lab Bead Solution is ready-to-use solution and is suitable for dilution and/or storage of plain, dyed, or functionalized polymer microspheres
Bangs Lab Bead Solution
SOLN1-500 500 ML
EUR 98.51
Description: Bangs Lab Bead Solution is ready-to-use solution and is suitable for dilution and/or storage of plain, dyed, or functionalized polymer microspheres
uv lamp 2x15w, 254/365nm
VL215-LC ea
EUR 817
uv lamp 2x6w, 254/365nm
VL6-LC ea
EUR 538
darkroom 254/365nm
VLCN15-LC ea
EUR 1535
CLIA kit for Human ON (Osteonectin)
E-CL-H0889 1 plate of 96 wells
EUR 584
  • Gentaur's ON CLIA kit utilizes the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ON . Standards or samples are added to the micro CLIA plate wells and combined with the spec
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Description: A sandwich CLIA kit for quantitative measurement of Human ON (Osteonectin) in samples from Serum, Plasma, Cell supernatant
CLIA kit for Mouse ON (Osteonectin)
E-CL-M0530 1 plate of 96 wells
EUR 584
  • Gentaur's ON CLIA kit utilizes the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ON . Standards or samples are added to the micro CLIA plate wells and combined with the spec
  • Show more
Description: A sandwich CLIA kit for quantitative measurement of Mouse ON (Osteonectin) in samples from Serum, Plasma, Cell supernatant
CLIA kit for Rat ON (Osteonectin)
E-CL-R0498 1 plate of 96 wells
EUR 584
  • Gentaur's ON CLIA kit utilizes the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ON . Standards or samples are added to the micro CLIA plate wells and combined with the specif
  • Show more
Description: A sandwich CLIA kit for quantitative measurement of Rat ON (Osteonectin) in samples from Serum, Plasma, Cell supernatant
ELISA kit for Human ON (Osteonectin)
E-EL-H1351 1 plate of 96 wells
EUR 534
  • Gentaur's ON ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ON. Standards or samples are added to the micro ELISA plate wells and combined with the sp
  • Show more
Description: A sandwich ELISA kit for quantitative measurement of Human ON (Osteonectin) in samples from Serum, Plasma, Cell supernatant
ELISA kit for Mouse ON (Osteonectin)
E-EL-M0867 1 plate of 96 wells
EUR 534
  • Gentaur's ON ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ON. Standards or samples are added to the micro ELISA plate wells and combined with the sp
  • Show more
Description: A sandwich ELISA kit for quantitative measurement of Mouse ON (Osteonectin) in samples from Serum, Plasma, Cell supernatant
ELISA kit for Rat ON (Osteonectin)
E-EL-R0701 1 plate of 96 wells
EUR 534
  • Gentaur's ON ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ON. Standards or samples are added to the micro ELISA plate wells and combined with the spec
  • Show more
Description: A sandwich ELISA kit for quantitative measurement of Rat ON (Osteonectin) in samples from Serum, Plasma, Cell supernatant
ELISA kit for Human ON (Osteonectin)
ELK1682 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteonectin (ON). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Osteonectin (ON).
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Description: A sandwich ELISA kit for detection of Osteonectin from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
ELISA kit for Rat ON (Osteonectin)
ELK1683 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteonectin (ON). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Osteonectin (ON).
  • Show more
Description: A sandwich ELISA kit for detection of Osteonectin from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
ELISA kit for Mouse ON (Osteonectin)
ELK1684 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteonectin (ON). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Osteonectin (ON).
  • Show more
Description: A sandwich ELISA kit for detection of Osteonectin from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
ELISA kit for Human Osteonectin (ON)
KTE60336-48T 48T
EUR 354
  • Osteonectin is a glycoprotein in the bone that binds calcium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone min
  • Show more
Description: Quantitative sandwich ELISA for measuring Human Osteonectin (ON) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human Osteonectin (ON)
KTE60336-5platesof96wells 5 plates of 96 wells
EUR 2252
  • Osteonectin is a glycoprotein in the bone that binds calcium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone min
  • Show more
Description: Quantitative sandwich ELISA for measuring Human Osteonectin (ON) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human Osteonectin (ON)
KTE60336-96T 96T
EUR 572
  • Osteonectin is a glycoprotein in the bone that binds calcium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone min
  • Show more
Description: Quantitative sandwich ELISA for measuring Human Osteonectin (ON) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
MS 245 oxalate
B5540-10 10 mg
EUR 292
MS 245 oxalate
B5540-5 5 mg
EUR 210
Methyl-PEG3-MS
abx186609-250mg 250 mg
EUR 439
  • Shipped within 1-2 weeks.
MS-275 (Entinostat)
E1KS1053 25 mg
EUR 247
Biotin-LC-Protein Kinase G Substrate; Biotin-LC-G-Subtide
5-00802 4 x 5mg Ask for price
Sulfo-NHS-LC-Biotin
2326-100
EUR 311
Sulfo-NHS-LC-Biotin
2326-50
EUR 202
Sulfo-NHS-LC-Biotin
2326-set
EUR 213
Polyclonal CD49e LC Antibody
APR05533G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human CD49e LC . This antibody is tested and proven to work in the following applications:
Complement C1R LC antibody
70R-49435 100 ul
EUR 244
Description: Purified Polyclonal Complement C1R LC antibody
Cathepsin D LC antibody
70R-49618 100 ul
EUR 244
Description: Purified Polyclonal Cathepsin D LC antibody
Cathepsin D LC Antibody
20-abx009376
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.
Complement C1R LC Antibody
20-abx009532
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
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Cathepsin C LC Antibody
20-abx121043
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
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CD41 LC 1 Antibody
20-abx121061
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
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Factor IX LC Antibody
20-abx121094
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
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Factor XI LC Antibody
20-abx121097
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.
Analysis of the 9 biomarkers measured with PEA and LC/MRM-MS displayed correlation coefficients >+0.6, related AUCs and DeLong’s p-values indicating no variations in AUCs for biomarkers like insulin-like development issue binding protein 2 (IGFBP2), matrix metalloproteinase 9 (MMP9) and serum paraoxonase lactonase 3 (PON3). Evaluating six proteins measured with PEA and QMA confirmed good correlation and related diagnostic efficiency for just one protein, development differentiation issue 15 (GDF15).

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